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Each CSM Single Drop-Out mixture contain all components except for one essential nutrient
discoideum in high cell densities
Besides these components
The antibiotic enters into sensitive cells by active transport
LoFlo Medium supplemented with Yeast extract Size:1kg Each CSM Single Drop-Out mixtureFormula g l Glucose 11 KH2PO4 0. 68 Casein Peptone 5 Yeast extract 0. 7 NH4Cl 26. 8 MgCl2 37. 1 CaCl2 1. 1 FeCl3 8. 11 Na2 EDTA 4. 84 ZnSO4 2. 30 H3BO3 1. 11 MnCl2. 4H2O 0. 51 CoCl2 0. 17 CuSO5. 5H2O 0. 15 (NH4)6M07O24. 4H2O 0. 1 Suspend 17. 5 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6. 5
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